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Determination of hippurate hydrolysis by gas-liquid chromatography
P L Wallace1, C M Patton, C W Moss
1Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
Journal of Clinical Microbiology
|September 1, 1987
Abstract:
A rapid gas-liquid chromatographic procedure was developed to determine hippurate hydrolysis by microorganisms. Bacterial cells were inoculated into 0.4 ml of 1% sodium hippurate and incubated for 2 h at 37 degrees C. Cells were removed by centrifugation, and the benzoate released by enzyme activity was converted to methyl benzoate and analyzed by gas-liquid chromatography. This procedure is sensitive, and its specificity provides a high degree of reliability for organisms with weak hippuricase activity.