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Updated: Aug 16, 2025

Optical Trapping of Nanoparticles

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Single-Particle Optical Imaging for Ultrasensitive Bioanalysis.

Yujie Liu¹, Binxiao Li², Baohong Liu²

¹Shanghai Institute of Pediatric Research, Shanghai Key Laboratory of Pediatric Gastroenterology and Nutrition, Xin Hua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.

|December 23, 2022

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Single-particle optical imaging offers ultrasensitive detection of low-abundance biomarkers in biofluids. This technique enhances early diagnosis and disease management by analyzing individual biomolecules with high sensitivity.

Area of Science:

Biomedical Engineering
Analytical Chemistry
Optical Physics

Background:

Quantitative detection of low-abundance biomarkers in biofluids is critical for early disease diagnosis and management.
Existing ensemble-sensing methods often lack the sensitivity required for ultralow-abundance biomolecule detection.
Single-particle imaging (SPI) techniques have emerged as powerful tools for analyzing individual biomolecules by leveraging unique properties of luminescent particles.

Purpose of the Study:

To review the latest advancements in single-particle optical imaging (OSPI) for ultrasensitive bioanalysis.
To highlight the applications of OSPI in basic biological studies and clinical diagnostics.
To discuss the challenges and future trends in OSPI-based bioanalysis.

Main Methods:

Keywords:

nanoparticle labels optical imaging single-particle imaging ultrasensitive analysis

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Survey of state-of-the-art OSPI techniques, including fluorescence, surface-enhanced Raman scattering (SERS), electrochemiluminescence (ECL), and dark-field scattering.
Emphasis on the role of metal and nonmetal nano-labels in improving signal-to-noise ratio (SNR) for OSPI.
Discussion of spatial-temporal measurement capabilities for biomarkers like proteins, nucleic acids, and extracellular vesicles (EVs) using single-entity sensitivity.

Main Results:

OSPI techniques, enhanced by various nano-labels, significantly improve SNR for detecting individual biomolecules.
Demonstrated single-entity sensitivity in spatial-temporal measurements of key biomarkers.
Successful application of OSPI in both fundamental biological research and clinical settings.

Conclusions:

OSPI provides a promising platform for ultrasensitive bioanalysis, crucial for early disease detection and personalized medicine.
Continued development of nano-labels and OSPI techniques will further enhance sensitivity and expand clinical applications.
Addressing current challenges in OSPI will pave the way for broader adoption in diagnostics and biological studies.