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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

500
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
500
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

503
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
503

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Related Experiment Video

Updated: Aug 16, 2025

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
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Antibiotic Susceptibility Testing with Raman Biosensing.

Andrei Novikov1, Adeliya Sayfutdinova1, Ekaterina Botchkova1

  • 1Department of Physical and Colloid Chemistry, Gubkin University, 65/1 Leninsky Prospect, 119991 Moscow, Russia.

Antibiotics (Basel, Switzerland)
|December 23, 2022
PubMed
Summary

Rapid antibiotic susceptibility testing is crucial for combating bacterial infections and antibiotic resistance. Raman spectroscopy offers a fast, selective method to detect bacterial responses to antibiotics, aiding in effective antimicrobial therapy.

Keywords:
antibiotic susceptibilityantibioticsbacteriaminimum inhibitory concentrationmultidrug resistancenanomaterialsnanoparticlessurface-enhanced Raman scattering

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Area of Science:

  • Biomedical Engineering
  • Spectroscopy
  • Microbiology

Background:

  • Antibiotics are vital for treating bacterial infections but widespread use drives antibiotic resistance.
  • Efficient antimicrobial therapy necessitates rapid detection of bacterial antibiotic susceptibility.
  • Antibiotic resistance poses a significant global health threat.

Purpose of the Study:

  • To review Raman spectroscopy-based optical biosensing approaches for antibiotic susceptibility testing.
  • To highlight the potential of Raman spectroscopy in addressing multidrug-resistant infections.
  • To analyze the advantages of Raman spectroscopy for rapid diagnostics.

Main Methods:

  • Analysis of Raman spectroscopy techniques for bacterial analysis.
  • Review of surface-enhanced Raman spectroscopy (SERS) and resonance Raman spectroscopy (RRS) applications.
  • Focus on optical biosensing for detecting antibiotic-induced cellular changes.

Main Results:

  • Raman spectroscopy detects antibiotic-induced physiological changes in bacteria via spectral features.
  • Enhanced sensitivity is achieved with SERS and RRS.
  • These spectral changes correlate with bacterial antibiotic susceptibility.

Conclusions:

  • Raman spectroscopy is a promising tool for rapid antibiotic susceptibility testing.
  • This technique can aid in personalized antimicrobial therapy, especially for resistant infections.
  • Optical biosensing with Raman spectroscopy offers a non-invasive and selective diagnostic approach.