Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:46

Epigenetic Regulation

31.3K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage.

Nature communications·2026
Same author

Written in blood: DNA methylation in liquid biopsy.

Nature reviews. Genetics·2026
Same author

Unraveling lncRNA diversity at a single cell resolution and in a spatial context across different cancer types.

Nature methods·2026
Same author

Neuro-Immune Axis in Trauma-Induced Heterotopic Ossification: Mechanisms and Therapeutic Implications.

Cells·2026
Same author

A novel non-invasive imaging technique for mapping microplastic accumulation and distribution in different organ systems of Indian major carp <i>Labeo rohita</i> (Ham. 1822).

Current research in toxicology·2026
Same author

Isoform-specific m6A deposition and coordinated splicing shape mammalian transcriptome evolution.

Nature communications·2026
Same journal

RETRACTED: Sabir et al. DNA Based and Stimuli-Responsive Smart Nanocarrier for Diagnosis and Treatment of Cancer: Applications and Challenges. <i>Cancers</i> 2021, <i>13</i>, 3396.

Cancers·2026
Same journal

Correction: Adeluola et al. Chemoprevention of 4-NQO-Induced Oral Cancer by the Combination of Resveratrol and EGCG: In Vivo, In Silico and In Vitro Studies. <i>Cancers</i> 2026, <i>18</i>, 1098.

Cancers·2026
Same journal

Correction: Peñalver et al. Guidelines for Diagnosis, Treatment, and Follow-Up of Patients with Follicular Lymphoma-Spanish Lymphoma Group (GELTAMO) 2026. <i>Cancers</i> 2026, <i>18</i>, 395.

Cancers·2026
Same journal

Correction: Accorsi Buttini et al. Development of a Simplified Geriatric Score-4 (SGS-4) to Predict Outcomes After Allogeneic Hematopoietic Stem Cell Transplantation in Patients Aged over 50. <i>Cancers</i> 2025, <i>17</i>, 3278.

Cancers·2026
Same journal

Age-Stratified Long-Term Outcomes of Immune Checkpoint Inhibitors for Stage IV Melanoma and NSCLC in The Netherlands: A Population-Based Study.

Cancers·2026
Same journal

Targeting Ferroptosis in Glioblastoma: Molecular Mechanisms, Tumor Microenvironment, and Therapeutic Opportunities.

Cancers·2026
See all related articles

Related Experiment Video

Updated: Aug 16, 2025

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

5.6K

Single-Cell DNA Methylation Analysis in Cancer.

Hannah O'Neill1, Heather Lee2,3, Ishaan Gupta4

  • 1Department of Pathology, Dunedin School of Medicine, University of Otago, Dunedin 9016, New Zealand.

Cancers
|December 23, 2022
PubMed
Summary
This summary is machine-generated.

Single-cell DNA methylation sequencing offers new insights into cancer biology by revealing cellular heterogeneity. This technology aids in identifying biomarkers and improving diagnostics, despite current challenges.

Keywords:
DNA methylationcancersingle cell

More Related Videos

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.7K

Related Experiment Videos

Last Updated: Aug 16, 2025

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

5.6K
LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles
12:18

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

5.8K
Methyl-binding DNA capture Sequencing for Patient Tissues
08:40

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

8.7K

Area of Science:

  • Epigenetics and Cancer Biology
  • Genomics and Molecular Biology

Background:

  • Cancer research traditionally analyzes bulk tissue, averaging epigenetic data and masking cellular heterogeneity.
  • Aberrant DNA methylation is crucial in cancer, affecting cell state, gene regulation, and stability.
  • Single-cell analysis overcomes limitations of bulk analysis for cancer research.

Purpose of the Study:

  • To explore the impact of single-cell sequencing on understanding cancer biology.
  • To discuss the potential clinical benefits of single-cell DNA methylation analysis.
  • To review the techniques, applications, and challenges of single-cell DNA methylation sequencing.

Main Methods:

  • Review of current literature on single-cell DNA methylation sequencing techniques.
  • Exploration of experimental approaches enabling single-cell methylome data acquisition.
  • Analysis of bioinformatic and practical challenges in the field.

Main Results:

  • Single-cell sequencing allows for the discovery of novel aberrant methylation patterns.
  • It enables the identification of cancer subpopulations and cell lineage tracing.
  • Advancements provide opportunities for biomarker discovery and improved diagnostics.

Conclusions:

  • Single-cell DNA methylation sequencing holds significant promise for advancing cancer research and clinical applications.
  • Overcoming technical and bioinformatic challenges is key to realizing its full potential.
  • This technology is poised to revolutionize our understanding of cancer heterogeneity.