Related Experiment Video
Updated: Aug 16, 2025

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
Published on: May 14, 2020
QTLs Related to Rice Callus Regeneration Ability: Localization and Effect Verification of qPRR3
Jiemin Wu1,2, Xinlei Chang1,2, Chuanhong Li1,2
1National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Wuhan 430070, China.
Abstract:
Mature and efficient tissue culture systems are already available for most japonica rice varieties (Oryza sativa ssp. geng). However, it remains challenging to regenerate the majority of indica rice varieties (Oryza sativa ssp. xian). In this study, quantitative trait loci (QTLs) associated with rice callus regeneration ability were identified based on the plant regeneration rate (PRR) and total green plant rate (TGPR) of the 93-11 × Nip recombinant inbred line population. Significant positive correlations were found between PRR and TGPR. A total of three QTLs (one for PRR and two for TGPR) were identified. qPRR3 (located on chromosome 3) was detected for both traits, which could explain 13.40% and 17.07% of the phenotypic variations of PRR and TGPR, respectively. Subsequently, the effect of qPRR3 on callus regeneration ability was validated by cryptographically tagged near-isogenic lines (NILs), and the QTL was narrowed to an interval of approximately 160 kb. The anatomical structure observation of the regenerated callus of the NILs revealed that qPRR3 can improve the callus regeneration ability by promoting the regeneration of shoots.
Related Concept Videos
Cell Signaling in Plants
Gene Regulation in Microbial Communities: Quorum Sensing
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

