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Cloning and Functional Characterization of SpZIP2.

Tian-Long Han¹, Ting-Wei Tang¹, Pei-Hong Zhang²

¹College of Agronomy, Hunan Agricultural University, Changsha 410128, China.

|December 23, 2022

Summary

The ZIP family gene SpZIP2 in the hyperaccumulator Sedum plumbizincicola plays a role in cadmium uptake. SpZIP2 enhances plant sensitivity to cadmium, contributing to its hyperaccumulation.

Keywords:

Cd accumulation Cd tolerance S. plumbizincicola ZIP2

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Area of Science:

Plant Biology
Environmental Science
Molecular Biology

Background:

Zinc (Zn)-regulated and iron (Fe)-regulated transporter-like proteins (ZIP) are crucial for metal accumulation in plants.
Sedum plumbizincicola is a known hyperaccumulator of cadmium (Cd) and Zn, but the specific roles of its ZIP family genes are not well understood.

Purpose of the Study:

To identify and characterize ZIP family genes in S. plumbizincicola.
To investigate the function of SpZIP2 in cadmium and zinc transport and accumulation.

Main Methods:

Transcriptome analysis to identify ZIP genes.
Gene cloning and functional analysis of SpZIP2 in yeast.
Metal sensitivity assays and ion content determination.
Subcellular localization studies.

Main Results:

Twelve ZIP family genes were identified in S. plumbizincicola, including SpZIP2.
SpZIP2 expression was higher in roots than shoots and responded to Cd stress.
SpZIP2 expression in yeast increased sensitivity to Cd and enhanced Cd accumulation.
SpZIP2 was localized to the plasma membrane and endoplasmic reticulum.

Conclusions:

SpZIP2 is involved in cellular cadmium uptake and accumulation.
SpZIP2 may contribute to the hyperaccumulation of cadmium in S. plumbizincicola.