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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Potentiometric MIP-Modified Screen-Printed Cell for Phenoxy Herbicides Detection.

Camilla Zanoni¹, Stefano Spina¹, Lisa Rita Magnaghi^1,2

¹Department of Chemistry, University of Pavia, Via Taramelli 12, 27100 Pavia, Italy.

International Journal of Environmental Research and Public Health

|December 23, 2022

Summary

A novel molecularly imprinted polymer (MIP) sensor detects the herbicide 2-methyl-4-chlorophenoxyacetic acid (MCPA). This MIP-based screen-printed cell offers precise environmental monitoring for MCPA contamination.

Keywords:

2-methyl-4-chlorophenoxyacetic acid (MCPA)MIP-based electrodes analytical chemistry electroanalysis herbicides molecularly imprinted polymers (MIP)potentiometric screen-printed cell screen-printed electrodes

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Published on: November 22, 2016

Area of Science:

Electroanalytical Chemistry
Polymer Science
Environmental Science

Background:

Phenoxy herbicides, like 2-methyl-4-chlorophenoxyacetic acid (MCPA), are widely used for weed control in agriculture.
Accurate detection of MCPA is crucial for environmental monitoring and ensuring crop safety.

Purpose of the Study:

To develop a molecularly imprinted polymer (MIP)-based screen-printed electrochemical cell for sensitive MCPA detection.
To evaluate the performance of the developed sensor for quantifying MCPA in environmental samples.

Main Methods:

Fabrication of a screen-printed potentiometric cell utilizing a graphite working electrode modified with a MIP film.
The MIP film was synthesized using 2-methyl-4-chlorophenoxyacetic acid (MCPA) as a template, methacrylic acid (MAA), and ethylene glycol dimethacrylate (EGDMA).
Electrochemical measurements were performed in a phosphate buffer at pH 5.5 to detect the deprotonated MCPA species.

Main Results:

A linear decrease in potential with MCPA concentration was observed in the range of 4 × 10^-8 to 1 × 10^-6 mol L^-1.
The sensor achieved a low detection limit of 10 nmol L^-1 and a limit of quantification of 40 nmol L^-1.
A Nernstian slope of approximately -59 mV/dec indicated efficient ion detection.

Conclusions:

The developed MIP-based screen-printed cell demonstrates high sensitivity and selectivity for MCPA detection.
The sensor's performance meets the precision and detection limits required for determining MCPA in contaminated environmental samples.
This technology offers a promising tool for effective environmental monitoring of phenoxy herbicides.