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Chrysin Induces Apoptosis via the MAPK Pathway and Regulates ERK/mTOR-Mediated Autophagy in MC-3 Cells

Gi-Hwan Jung1, Jae-Han Lee1, So-Hee Han1

  • 1Department of Companion and Laboratory Animal Science, Kongju National University, Daehak-ro, Yesan-gun 32439, Chungcheongnam-do, Republic of Korea.

International Journal of Molecular Sciences
|December 23, 2022
PubMed

Insights

Chrysin triggers apoptosis and autophagy in oral cancer cells, potentially offering a new therapeutic strategy. Induced autophagy provides a protective effect against cell death, highlighting chrysin

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cancer Research

Background:

  • Chrysin, a flavonoid from honey and phytochemicals, shows anticancer properties.
  • Its effects on oral cancer and the role of autophagy remain unclear.

Purpose of the Study:

  • Investigate chrysin-induced apoptosis and autophagy in human mucoepidermoid carcinoma (MC-3) cells.
  • Elucidate the underlying molecular mechanisms, including the MAPK pathway.

Main Methods:

  • Cell viability assays (MTT, DAPI, Annexin V/PI staining).
  • Western blotting for apoptosis and MAPK pathway proteins.
  • Acridine orange staining for autophagy detection.

Main Results:

  • Chrysin induced apoptosis and autophagy in MC-3 oral cancer cells.
  • The MAPK/ERK pathway was involved in chrysin's effects.
  • Autophagy exhibited a cytoprotective role against apoptosis.

Conclusions:

  • Chrysin effectively induces apoptosis and autophagy in oral cancer cells.
  • The interplay between apoptosis and autophagy suggests therapeutic potential.
  • Further research into chrysin as an oral cancer treatment is warranted.
Keywords:
apoptosisautophagychrysinmucoepidermoid carcinomaoral cancer

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