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Nephroprotective Activity of Papaloquelite (Porophyllum ruderale) in Thioacetamide-Induced Injury Model

María José Vázquez-Atanacio1,2, Mirandeli Bautista2, Manasés González-Cortazar3

  • 1Área Académica de Medicina Veterinaria y Zootecnia, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km 1, Ex-Hda. de Aquetzalpa, Tulancingo 43600, Hidalgo, Mexico.

Plants (Basel, Switzerland)
|December 23, 2022
PubMed

Insights

The Mexican plant Porophyllum ruderale (HEPr) exhibits significant antioxidant properties and protects against acute kidney injury in rats. Its compounds may offer therapeutic potential for kidney health.

Area of Science:

  • Pharmacology and Toxicology
  • Natural Product Chemistry
  • Nephrology

Background:

  • Acute kidney injury (AKI) is a critical condition linked to poor patient outcomes.
  • Traditional Mexican medicine utilizes Porophyllum ruderale for various ailments.
  • Understanding the nephroprotective potential of P. ruderale is crucial.

Purpose of the Study:

  • To evaluate the nephroprotective effects of a hydroalcoholic extract from Porophyllum ruderale (HEPr).
  • To assess the in vitro antioxidant and anti-inflammatory activities of HEPr.
  • To determine the in vivo efficacy of HEPr in a rat model of kidney injury.

Main Methods:

  • In vitro assays for antioxidant (FRAP, DPPH, ABTS) and anti-inflammatory (LPS-NO) activity.
  • In vivo study using a thioacetamide-induced acute kidney injury model in rats.
  • Analysis of kidney injury biomarkers in urine and serum; High-Performance Liquid Chromatography (HPLC) for compound identification.

Main Results:

  • HEPr demonstrated high antioxidant capacity and moderate anti-inflammatory effects.
  • Rats treated with HEPr maintained normal levels of urinary and serum kidney injury biomarkers.
  • HPLC analysis identified major compounds including caffeoylquinic acids, ferulic acid, and quercetin/kaempferol glucosides.

Conclusions:

  • Porophyllum ruderale extract (HEPr) exhibits significant nephroprotective effects.
  • The plant's antioxidant properties contribute to its kidney-protective action.
  • HEPr is a promising source of natural compounds for managing acute kidney injury.
Keywords:
Porophyllum ruderaleanti-inflammatoryantioxidantnephroprotective

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