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Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Related Experiment Video

Updated: Jun 23, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

Procyanidins from Cranberry Press Residues-Extraction Optimization, Purification and Characterization.

Linards Klavins¹, Ingus Perkons², Marcis Mezulis¹

¹Department of Environmental Science, University of Latvia, LV-1004 Riga, Latvia.

Plants (Basel, Switzerland)

|December 23, 2022

Summary

American cranberries are a rich source of Type A procyanidins, potent antioxidants. Optimized extraction from cranberry waste yields valuable nutraceuticals with significant health benefits.

Keywords:

American cranberry Vaccinium macrocarpon antioxidants optimization press residues procyanidins response surface methodology valorization

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Last Updated: Jun 23, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

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Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

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Published on: May 27, 2019

Area of Science:

Food Chemistry
Nutraceuticals
Natural Products

Background:

Procyanidins, polyphenols found in foods like cranberries, exhibit antioxidant, anticancer, and anti-inflammatory properties.
American cranberries are a significant source of Type A procyanidins, utilized for preventing urinary tract infections.
Cranberry processing for juice generates press residues rich in extractable procyanidins.

Purpose of the Study:

To optimize the extraction of procyanidins from American cranberry press residues.
To purify and fractionate extracted procyanidins.
To evaluate the antioxidative potential and characterize individual procyanidins in the fractions.

Main Methods:

Response Surface Methodology (RSM) for extraction optimization.
Acetone-based extraction without acidifying agents.
Liquid Chromatography-Electrospray Ionization-Fourier Transform Ion Cyclotron Resonance-High Resolution Mass Spectrometry (LC-ESI-FTICR-HRMS) for analysis.
DPPH, ABTS, and FRAP assays for antioxidative activity assessment.

Main Results:

Optimal extraction achieved using 53% acetone without an acidifying agent.
A strong correlation was found between procyanidin content and antioxidative activity.
Purified fractions contained 78 individual procyanidins, including 65 Type A, with a polymerization degree up to 9.

Conclusions:

Cranberry press residues are a valuable source of Type A procyanidins with significant antioxidative potential.
Optimized extraction methods can yield high-quality nutraceuticals from food waste.
Further research into these fractions could lead to novel health applications.