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Single Camera-Based Dual-Channel Near-Infrared Fluorescence Imaging system
Janghoon Choi1,2, Jun-Geun Shin3, Yoon-Oh Tak1
1Intelligent Photonic IoT Research Center, Korea Photonics Technology Institute, Gwangju 61007, Republic of Korea.
Sensors (Basel, Switzerland)
|December 23, 2022
Summary
This study introduces a compact, single-camera near-infrared (NIR) fluorescence imaging system. It enables real-time dual-channel NIR and color imaging, reducing surgical equipment bulk and cost.
Area of Science:
- Medical Imaging
- Biophotonics
- Surgical Technology
Background:
- Conventional dual-channel near-infrared (NIR) fluorescence imaging systems often require multiple cameras and optical components.
- This setup leads to increased equipment size, restricted surgical maneuverability, and higher manufacturing costs.
- A need exists for more compact and cost-effective fluorescence imaging solutions in surgical settings.
Purpose of the Study:
- To develop a novel, single-camera dual-channel NIR fluorescence imaging system.
- To simultaneously acquire color and dual-channel NIR fluorescence images in real time.
- To reduce the physical footprint and cost of fluorescence imaging equipment for surgical applications.
Main Methods:
- Utilized a single camera with two image sensors for simultaneous color and single-channel NIR fluorescence acquisition.
- Implemented time-division multiplexing for two channels synchronized with two excitation lasers.
- Employed image processing techniques to mitigate crosstalk effects between fluorescent agents.
Main Results:
- Successfully reduced the volume of the image acquisition unit compared to multi-camera systems.
- Demonstrated real-time acquisition of color and dual-channel NIR fluorescence images.
- Evaluated system performance, including sensitivity, crosstalk, and signal-to-background ratio for two fluorescent agents.
Conclusions:
- The proposed single-camera system offers a compact and cost-effective alternative for dual-channel NIR fluorescence imaging.
- Reduced equipment size improves usability during surgical procedures, minimizing movement obstruction.
- This technological advancement has the potential to increase the adoption of advanced fluorescence imaging in clinical and animal studies.
Keywords:
dual-channel fluorescence imagingindocyanine greenmethylene bluenear-infrared fluorescence imaging systemreal-time imagingMore Related Videos
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