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Updated: Aug 16, 2025
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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
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High APOBEC3B mRNA Expression Is Associated with Human Papillomavirus Type 18 Infection in Cervical Cancer
Gisele R de Oliveira1, Pedro S Carvalho1, Valdimara C Vieira2
1Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil.
Viruses
|December 23, 2022
Summary
High expression of Apolipoprotein B (A3B) is linked to HPV18-positive cervical cancer. This finding suggests A3B may serve as a biomarker for HPV infection and a prognostic tool for patient outcomes.
Area of Science:
- Molecular Biology
- Oncology
- Virology
Background:
- APOBEC3 (A3) proteins are cytidine deaminases involved in DNA/RNA mutation.
- APOBEC3B (A3B) is a DNA mutagen with elevated expression in various cancers.
- The role of A3B in HPV infection and cervical cancer remains unclear.
Purpose of the Study:
- To investigate the association between A3B expression levels and HPV types in cervical cancer patients.
- To determine if A3B expression can serve as a biomarker or prognostic indicator in cervical cancer.
Main Methods:
- Tumor biopsies from 216 cervical cancer patients were analyzed for HPV type.
- A3B expression levels were quantified using real-time quantitative PCR on RNA extracted from biopsies.
Main Results:
- Median A3B expression was significantly higher in HPV18-positive samples compared to HPV16-positive and other HPV types.
- This upregulation was also observed in squamous cell carcinoma, particularly in HPV18-positive cases.
Conclusions:
- A3B expression is differentially upregulated in cervical cancer associated with HPV18 infection.
- A3B shows potential as a biomarker for HPV infection and a prognostic tool for cervical cancer clinical outcomes.
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