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Mitochondrial H2S donor AP39 induces stomatal closure by modulating guard cell mitochondrial activity
Rosario Pantaleno1, Denise Scuffi1, Alex Costa2
1Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas, Mar del Plata 7600, Argentina.
Plant Physiology
|December 23, 2022
Summary
Mitochondria-targeted hydrogen sulfide (H2S) donors trigger stomatal closure in plants by influencing guard cell mitochondrial activity and energy status. This research highlights the crucial role of mitochondria in H2S-mediated stomatal regulation.
Area of Science:
- Plant Physiology
- Mitochondrial Biology
- Biochemistry
Background:
- Hydrogen sulfide (H2S) acts as a crucial gasotransmitter in plant physiological processes.
- Guard cells regulate stomatal pores for gas exchange, possessing high mitochondrial activity.
- The specific role of mitochondrial activity in stomatal closure remains unclear.
Purpose of the Study:
- To investigate the effect of mitochondrial-targeted H2S on stomatal closure.
- To elucidate the involvement of mitochondrial activity in H2S-induced stomatal closure.
Main Methods:
- Utilized a mitochondrial-targeted H2S donor (AP39).
- Employed inhibitors of the mitochondrial electron transport chain (mETC) and cytochrome c (CYTc) mutants.
- Applied fluorescent probes and genetically encoded biosensors to monitor mitochondrial potential and cellular redox state.
Main Results:
- AP39 induced dose-dependent stomatal closure.
- Mitochondrial CYTc and complex IV activity were essential for AP39's effect.
- AP39 application led to mitochondrial hyperpolarization, increased cytosolic ATP and H2O2, and altered glutathione redox state in guard cells.
Conclusions:
- Mitochondria-targeted H2S donors effectively induce stomatal closure.
- Guard cell mitochondrial activity, mETC function, and cellular energy/redox balance are modulated by H2S.
- Establishes a link between mitochondrial H2S, mitochondrial function, and stomatal closure regulation.
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