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Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Competitive Ratiometric Aptasensing with Core-Internal Standard-Shell Structure Based on Surface-Enhanced Raman

Wenya Wei¹, Md Mehedi Hassan¹, Jizhong Wu¹

¹School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu212013, P.R. China.

Journal of Agricultural and Food Chemistry

|December 23, 2022

Summary

This study introduces a self-calibrating surface-enhanced Raman scattering (SERS) aptasensor for accurate quantification. The novel ratiometric method enhances reproducibility and stability in detecting targets like okadaic acid (OA).

Keywords:

core−shell nanoparticles magnetic microspheres okadaic acid ratiometric surface-enhanced Raman scattering

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Area of Science:

Nanotechnology
Biosensing
Analytical Chemistry

Background:

Reproducibility and stability are crucial for quantitative surface-enhanced Raman scattering (SERS) methods.
Developing self-calibrating SERS substrates is essential for reliable target quantification.

Purpose of the Study:

To develop a competitive ratiometric SERS aptasensor for enhanced quantification.
To improve the reproducibility and stability of SERS-based sensing for targets such as okadaic acid (OA).

Main Methods:

A ratiometric SERS aptasensor was constructed using 4-aminothiophenol as an internal standard (IS).
Aptamers and methylene blue-functionalized complementary DNA (MB-cDNA) were loaded onto the substrate.
Magnetic separation was employed for target recognition and MB-cDNA shedding, enabling ratiometric determination.

Main Results:

The aptasensor demonstrated a negative correlation between ratiometric intensity and OA concentration (0.5-100 ng/mL).
The ratiometric strategy significantly improved sensing reproducibility and stability.
The method was successfully applied to detect OA in food and environmental samples.

Conclusions:

The developed ratiometric SERS aptasensor offers a reliable and stable method for quantitative target detection.
The magnetic separation and ratiometric strategies simplify aptasensor production and enhance performance.
This approach is adaptable for detecting various other targets.