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Related Experiment Videos

Development of mouse embryos cryopreserved by vitrification.

W F Rall1, M J Wood, C Kirby

  • 1MRC Experimental Embryology and Teratology Unit, Carshalton, Surrey, U.K.

Journal of Reproduction and Fertility
|July 1, 1987
PubMed
Summary

Vitrification of mouse embryos using a 90% cryoprotectant solution (90% VS1) resulted in high in vitro development and live births. This simple, rapid vitrification method successfully produced normal fetuses and young from cryopreserved embryos.

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Cryobiology

Background:

  • Cryopreservation of mammalian embryos is crucial for assisted reproductive technologies.
  • Vitrification offers a promising alternative to slow freezing, minimizing ice crystal formation.
  • Optimizing cryoprotective agent (CPA) concentrations is key for successful embryo vitrification.

Purpose of the Study:

  • To evaluate the efficacy of a novel vitrification solution (VS1) for cryopreserving eight-cell mouse embryos.
  • To compare the developmental potential of embryos vitrified in a full-strength VS1 versus a diluted 90% VS1 solution.
  • To establish a simple and rapid method for producing viable offspring from cryopreserved embryos.

Main Methods:

  • Eight-cell mouse embryos were exposed in three steps to either stock VS1 or 90% VS1 solutions.

Related Experiment Videos

  • Embryo suspensions were vitrified by rapid cooling in liquid nitrogen.
  • Vitrified embryos were assessed for in vitro development and subsequent transfer to pseudopregnant recipients.

    • Main Results:

    • Vitrification in 90% VS1 yielded 80% in vitro development and 17% live offspring (98 normal fetuses/young).
    • Vitrification in stock VS1 resulted in 22% in vitro development and only one live offspring.
    • The 90% VS1 solution prevented crystallization upon cooling, forming a glassy solid.

    Conclusions:

    • A simple and rapid vitrification method using 90% VS1 is effective for cryopreserving mouse embryos.
    • This technique significantly improves the success rate of producing normal offspring from cryopreserved embryos.
    • Optimized CPA concentrations in vitrification solutions are critical for successful embryo cryopreservation and subsequent development.