Related Experiment Video
Updated: Aug 16, 2025

The Caco-2 Cell Bioassay for Measurement of Food Iron Bioavailability
Published on: April 28, 2022
Mineral and Trace Elements in Nutritious Flours: Total Contents, In Vitro Bioaccessibility and Contribution to
Thaís L Souza1, Laís A Souza2, Isa S Barbosa1
1Instituto de Química, Universidade Federal da Bahia, Campus Universitário de Ondina, Ondina, Salvador, Bahia, 40170-115, Brazil.
Nutritious flours offer essential minerals like copper (Cu), iron (Fe), and zinc (Zn). This study assessed their total content and in vitro bioaccessibility, finding low bioaccessible fractions for these key minerals.
Area of Science:
- Food Science
- Nutritional Science
- Analytical Chemistry
Background:
- Increasing demand for healthier diets emphasizes the importance of nutrient intake from alternative sources like flours.
- Assessing total nutrient content and bioaccessibility is crucial for understanding actual nutrient or contaminant intake from food.
- Nutritious flours derived from fruits, vegetables, and cereals are gaining popularity as healthier dietary options.
Purpose of the Study:
- To determine the total content of essential minerals (Cu, Fe, Zn) in nutritious flours.
- To estimate the bioaccessible fraction of Cu, Fe, and Zn using an in vitro gastrointestinal model.
- To analyze mineral profiles and their relationships using multivariate statistical methods.
Main Methods:
- Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) was employed for elemental analysis after microwave acid digestion.
- The Unified Bioaccessible Method (UBM) was utilized for in vitro assessment of mineral bioaccessibility across gastric and gastrointestinal phases.
- Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were applied for data interpretation.
Main Results:
- Concentrations (μg g⁻¹) varied: Ca (341-6275), K (2984-13,953), P (476-6110), Na (<0.39-2995), Fe (1.4-167), Cu (<0.01-59.6), and Zn (<0.07-30.3).
- Bioaccessible fractions for Cu, Fe, and Zn were generally low, ranging from 0.96% to 40% in the gastrointestinal phase.
- Mineral data exhibited a tendency to form three distinct groups based on PCA and HCA.
Conclusions:
- The study quantified key mineral content in nutritious flours and evaluated their in vitro bioaccessibility.
- Low bioaccessibility of Cu, Fe, and Zn suggests potential limitations in nutrient absorption from these flour sources.
- Understanding mineral profiles and bioaccessibility is vital for optimizing the nutritional benefits of alternative food sources.
More Related Videos
04:54Assessing Mineral Availability in Fish Feeds using Complementary Methods Demonstrated with the Example of Zinc in Atlantic Salmon
Published on: October 29, 2021
05:55High-throughput, Microscale Protocol for the Analysis of Processing Parameters and Nutritional Qualities in Maize Zea mays L.
Published on: June 16, 2018
Related Concept Videos
Key Elements for Plant Nutrition
Minerals
Major...
Essential Minerals for Bone Health
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Mineral, Vitamin and Water Absorption
Bioavailability: Overview
Factors Influencing Bioavailability: First-Pass Elimination