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Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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Related Experiment Video

Updated: Jun 14, 2026

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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Guidelines for mouse and human DC functional assays.

Björn E Clausen1,2, Lukas Amon3, Ronald A Backer1,2

  • 1Research Center for Immunotherapy (FZI), University Medical Center of the Johannes-Gutenberg University Mainz, Mainz, Germany.

European Journal of Immunology
|December 23, 2022
PubMed
Summary
This summary is machine-generated.

This guide details methods for analyzing mouse and human dendritic cells (DC). It covers preparation, flow cytometry, microscopy, and functional assays like endocytosis, metabolism, migration, and antigen presentation for DC immunologists.

Keywords:
T cell responsedendritic cellendocytosislangerhans cellmigration

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Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Dendritic cells (DC) are crucial immune cells with diverse subsets.
  • Recent research highlights phenotypically distinct conventional DC (cDC) subsets with specialized functions.
  • Understanding cDC function requires robust and standardized analytical methods.

Purpose of the Study:

  • To provide state-of-the-art protocols for the comprehensive functional characterization of mouse and human dendritic cells (DC).
  • To offer guidelines for analyzing cDC subsets from various tissues.
  • To serve as an essential resource for basic and clinical DC immunologists.

Main Methods:

  • Preparation and phenotype analysis of DC via flow cytometry.
  • Functional assays including endocytosis, metabolism, and migration (in vitro, ex vivo, in vivo).
  • Transcriptomic, proteomic, inflammasome, and antigen presentation analyses of cDC populations.

Main Results:

  • Detailed protocols for assessing various functional aspects of cDC are presented.
  • Methods cover a wide range of cDC activities, from basic cellular processes to complex immune functions.
  • The guidelines facilitate the study of tissue- and context-dependent cDC specialization.

Conclusions:

  • This collection of peer-reviewed protocols offers a comprehensive resource for DC research.
  • Standardized methods enable reliable functional characterization of mouse and human cDC.
  • The guidelines support advancements in both basic and clinical immunology research involving dendritic cells.