Electrochemical performance of Paenibacillus profundus YoMME encapsulated in alginate polymer

Gram-positive bacterium Paenibacillus profundus YoMME, entrapped in an alginate polymer onto graphite paper, preserves its extracellular electron transfer capabilities. A current density of up to 30 mA m^-2 was generated at an applied potential of -200 mV (vs. SHE). Fivefold higher initial current density values were recorded at applied potentials of +220 mV and +600 mV. The electrochemical behavior of the encapsulated bioelectrodes has been also explored by cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy and evaluated by the parameters of the best-fitted equivalent electric circuit model. Over time the bacteria grow and divide within the alginate matrix, which affects considerably the capacitance and the charge transfer resistance of the coating. The impedance spectra follow the dynamics of the bacterial culture development within the alginate polymer and are useful for the prediction of the bioelectrode performance. A current density of 150 mA m^-2 was achieved when the alginate was functionalized by a mixture of the redox dyes thiazolyl blue (MTT) formazan and phenazine methosulfate (PMS). It is supposed that the added artificial mediators facilitate the electron transfer from the bacteria to the electrode surface by forming conductive cascade conduits through the alginate matrix.