Genistein microparticles prepared by antisolvent recrystallization with low-speed homogenization process

Area of Science:

• Materials Science
• Chemical Engineering
• Pharmacology

Background:

• Genistein (GMP) is a valuable compound with therapeutic potential.
• Conventional methods for particle size reduction can be energy-intensive and less efficient.
• Improving the bioavailability and solubility of genistein is crucial for its applications.

Purpose of the Study:

• To develop and optimize a novel antisolvent recrystallization technique for genistein particle formation.
• To characterize the physical and chemical properties of the resulting genistein particles.
• To evaluate the enhanced dissolution rate and antioxidant activity of the prepared genistein particles.

Main Methods:

• Antisolvent recrystallization technique optimized using a response surface approach.
• Single factor tests to determine optimal parameters: liquid-to-liquid ratio (9), solution concentration (21 mg/mL), nozzle diameter (700 μm), feed rate (39.65 mL/min), and homogenization rate (1500 rpm).
• Scanning Electron Microscopy (SEM) for morphology, thermal analysis, and infrared imaging for characterization.

Main Results:

• Achieved a minimum mean particle size of 202.782 nm.
• The prepared genistein particles exhibited improved dissolution rate and enhanced antioxidant activity compared to raw genistein powder.
• The novel method demonstrated successful particle size reduction and improved bioavailability.

Conclusions:

• The optimized antisolvent recrystallization technique offers an effective and energy-efficient method for producing nano-sized genistein particles.
• This approach significantly enhances genistein's dissolution, antioxidant properties, and bioavailability.
• The developed method holds promise for real-world applications in pharmaceuticals and nutraceuticals.