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Updated: Aug 16, 2025
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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Published on: May 17, 2014
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The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease
Marino Caruso1, Sébastien Meurant1, Damien Detraux1
1Laboratory of Biochemistry and Cell Biology (URBC), Namur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur), Namur, Belgium.
Stem Cell Reports
|December 23, 2022
Summary
Stem cell differentiation involves complex protein synthesis changes. This study reveals global translation repression during liver cell development, with specific gene rewiring for hepatospecific functions.
Area of Science:
- Molecular Biology
- Stem Cell Biology
- Proteomics
Background:
- Translational regulation is crucial for stem cell differentiation and proteome remodeling.
- Understanding these changes is key to controlling cell fate and function.
Purpose of the Study:
- To characterize translational remodeling during hepatogenic differentiation of induced pluripotent stem cells (iPSCs).
- To investigate global and transcript-specific changes in protein synthesis during liver cell development.
Main Methods:
- Polysome profiling was employed to analyze translational activity.
- Quantification of protein components of the translation machinery was performed.
Main Results:
- Protein synthesis initially increases upon exit from pluripotency, then globally decreases during hepatogenic maturation.
- Global translation efficiency of terminal oligopyrimidine tract (TOP) mRNAs declines.
- Key hepatogenic genes and transcripts for hepatospecific functions remain efficiently translated or are induced.
Conclusions:
- Hepatogenic differentiation involves a global decrease in protein synthesis.
- Specific translational rewiring of hepatospecific transcripts occurs alongside global repression.
Keywords:
LARP1TOP mRNAhepatocyte differentiationiPSCpolysome profilingstem cellstranslational regulationMore Related Videos
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