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BmNPV circular RNA-encoded peptide VSP39 promotes viral replication.

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This study identifies a viral circRNA (circRNA-000010) from Bombyx mori Nucleopolyhedrovirus that enhances viral replication. This circular RNA also encodes a small peptide, VSP39, which further promotes virus proliferation.

Keywords:
BmNPVVSP39Viral circRNA-010Viral replicationcircRNA

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Area of Science:

  • Virology
  • Molecular Biology
  • Genomics

Background:

  • Circular RNAs (circRNAs) are stable RNA molecules with crucial biological roles.
  • Research on circRNAs primarily focuses on endogenous forms, with limited investigation into virus-encoded circRNAs.

Purpose of the Study:

  • To identify and characterize viral circRNAs encoded by Bombyx mori Nucleopolyhedrovirus (BmNPV).
  • To investigate the role of a novel viral circRNA, circRNA-000010, in viral replication.
  • To explore the coding potential and function of circRNA-000010 and its translated products.

Main Methods:

  • circRNA-sequencing to identify viral circRNAs.
  • Reverse transcription PCR, rolling circle amplification, in situ hybridization, immunofluorescent staining, and Northern blotting for validation.
  • Overexpression and knockdown experiments to assess functional impact.
  • Peptide identification and functional assays.

Main Results:

  • Identification and confirmation of a viral circRNA, circRNA-000010, from BmNPV.
  • Demonstration that circRNA-000010 significantly promotes BmNPV replication.
  • Discovery of a viral small peptide, VSP39, translated from circRNA-000010.
  • Confirmation that VSP39 enhances viral replication.

Conclusions:

  • Viral circRNAs can encode functional peptides that promote viral replication.
  • circRNA-000010 and its peptide VSP39 represent novel factors in BmNPV pathogenesis.
  • Findings offer new insights into BmNPV genome coding capacity and host-virus interactions.