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Establishment of measles virus receptor-expressing Vero cells lacking functional poliovirus receptors

Kenji Someya1,2, Yuko Okemoto-Nakamura3, Takako Kurata4

  • 1Department of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.

Microbiology and Immunology
|December 23, 2022
PubMed

Insights

New cell lines aid global measles and rubella virus surveillance. These poliovirus-resistant cells efficiently support measles virus (MeV) and rubella virus (RuV) isolation, crucial for eradication efforts.

Area of Science:

  • Virology
  • Cell Biology
  • Public Health

Background:

  • Global initiatives aim to eliminate measles and rubella.
  • Active viral surveillance is critical for achieving these eradication goals.
  • The World Health Organization has issued guidelines to prevent poliovirus (PV) reintroduction.

Purpose of the Study:

  • To develop novel cell lines for enhanced viral surveillance.
  • To create a cell line resistant to poliovirus (PV) for safer handling of potentially infectious materials.
  • To support global efforts in measles virus (MeV) and rubella virus (RuV) elimination.

Main Methods:

  • Development of new cell lines (Vero ΔPVR1/2 hSLAM+) engineered for poliovirus (PV) non-susceptibility.
  • Testing the susceptibility of the new cell lines to measles virus (MeV) and rubella virus (RuV).
  • Evaluating the replication efficiency of MeV and RuV in the developed cell lines.

Main Results:

  • Successfully established poliovirus (PV)-non-susceptible cell lines.
  • Demonstrated efficient replication of measles virus (MeV) and rubella virus (RuV) in the new cell lines.
  • Confirmed the absence of poliovirus (PV) replication in these specialized cells.

Conclusions:

  • The novel Vero ΔPVR1/2 hSLAM+ cell lines are highly effective for isolating measles virus (MeV) and rubella virus (RuV).
  • These cell lines provide a safe and efficient platform for viral surveillance, supporting global MeV and RuV elimination goals.
  • The PV-non-susceptible nature of the cell lines mitigates risks associated with PV, aligning with WHO guidelines for PV eradication.
Keywords:
SLAMmeasles viruspoliovirusreceptorrubella virus

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