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Updated: Aug 16, 2025

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
Published on: May 21, 2019
Discovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana
M Arasimowicz-Jelonek1, J Floryszak-Wieczorek2, S Suarez3,4
1Department of Plant Ecophysiology, Adam Mickiewicz University, Poznań, Poland. magdalena.arasimowicz@amu.edu.pl.
Abstract:
Nitroxyl (HNO) is the one-electron reduced and protonated congener of nitric oxide (•NO), owning a distinct chemical profile. Based on real-time detection, we demonstrate that HNO is endogenously formed in Arabidopsis. Senescence and hypoxia induce shifts in the redox balance, triggering HNO decay or formation mediated by non-enzymatic •NO/HNO interconversion with cellular reductants. The stimuli-dependent HNO generation supports or competes with •NO signalling, depending on the local redox environment.
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