Molecular Mechanisms Associated with Protecting IEC-6 Cells from Acrylamide-Induced Tight Junction Damage by
Jiawen Lu1, Ying Yang1, Elisabeth Varga2
1State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, P. R. China.
Scope:
The previous in vivo studies show Ganoderma atrum polysaccharide (PSG-F2 ) has a protective effect against the acrylamide (AA)-induced intestinal oxidative damage in rats. Now, this study aims to explore the protective mechanism with IEC-6 cell model.
Methods And Results:
Based on RNA Sequencing (RNA-Seq), the study screens MAPK signaling pathway as one of the most crucial pathways for pretreatment with PSG-F2 against AA-induced damage in IEC-6 cells. In total, six key MAPK signaling pathway-related proteins (p-P38/P38, p-ERK/ERK, and p-JNK/JNK), and three tight junction key proteins (Zonula Occludens protein-1, Claudin-1, and Occludin) are detected by Western blot and immunofluorescence, which verify the RNA-Seq data. Moreover, PD98059 interference inhibits critical proteins in the MAPK signaling pathway, thus uncovering the precise molecular mechanisms of MAPK/ERK signaling pathway involve in the protective effects of PSG-F2 against AA-induced intestinal barrier damage.
Conclusion:
These findings confirm that PSG-F2 can be used as a daily dietary supplement to protect the intestinal cells from damage caused by thermal processing hazards AA.
