Related Experiment Video

Updated: Aug 16, 2025

RhoC GTPase Activation Assay
09:58

RhoC GTPase Activation Assay

Published on: August 22, 2010

12.5K

SGOL2 promotes prostate cancer progression by inhibiting RAB1A ubiquitination

Tingting Lv1, Dongwei He1, Xiaokuan Zhang1

  • 1Department of Immuno-Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, P.R. China.

Aging
|December 24, 2022
PubMed

Insights

SGOL2 stabilizes RAB1A expression by inhibiting its ubiquitination, promoting prostate cancer cell proliferation and migration. These proteins are crucial for modulating the tumor microenvironment in advanced prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Prostate cancer, particularly castration-resistant prostate cancer (CRPC), presents a significant clinical challenge with limited effective treatments.
  • SGOL2 is an emerging molecule implicated in cancer cell proliferation.

Purpose of the Study:

  • To investigate the role of SGOL2 in prostate cancer progression.
  • To elucidate the regulatory mechanism between SGOL2 and RAB1A in prostate cancer cell lines.

Main Methods:

  • Mass spectrometry and Co-immunoprecipitation (Co-IP) to validate protein-protein interactions.
  • In vitro studies using prostate cancer cell lines to assess proliferation and migration.
  • Ubiquitination assays and rescue experiments to confirm regulatory pathways.

Main Results:

  • SGOL2 interacts with RAB1A at a protein-protein level.
  • SGOL2 inhibits RAB1A ubiquitination, leading to its stabilization.
  • Upregulation of RAB1A by SGOL2 promotes prostate cancer cell proliferation and migration.
  • SGOL2 and RAB1A play a role in modulating the tumor microenvironment (TME).

Conclusions:

  • SGOL2 stabilizes RAB1A expression, thereby promoting prostate cancer development.
  • Both SGOL2 and RAB1A are critical for tumor microenvironment modulation in prostate cancer.
Keywords:
RAB1ASGOL2prostate cancertumor microenvironmentubiquitination

More Related Videos

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells09:40

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Published on: November 2, 2017

7.4K
An Orthotopic Murine Model of Human Prostate Cancer Metastasis06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

35.3K

Related Concept Videos

Rab Proteins01:14

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.0K
Rab Cascades01:25

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.7K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.4K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
4.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K