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Metabolomics uncovers adaptation discrepancy among anammox granular sludge with different granule size: Metabolic
Chenyang Ma1, Wei Zeng1, Jianmin Li1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Department of Environmental Engineering, Beijing University of Technology, Beijing 100124, China.
Abstract:
The relationship between granular size and anaerobic ammonium oxidation (anammox) performance in the anammox granular sludge (AnGS) system has been extensively observed. However, the metabolic pathways regulated by communication and cross-feedings among anammox consortia remain unclear. The reactor operation and metabolomics analyses were combined to explore the influence of microbiota cooperation on metabolic pathways and granule properties under low temperature (18 °C) and nitrite inhibition. Anammox activity was sustained under challenging circumstances by active quorum sensing among anammox consortia in AnGS with diameters larger than 1.4 mm, which promoted nucleotide metabolism. Cross-feedings among anammox consortia increased the levels of molybdopterin cofactor and folate meanwhile decreasing the cost of carbon fixation metabolism, which supported anabolism and maintained the content of heme c and extracellular polymeric substance. These metabolic insights into the AnGS system provide a new view for anammox process overcoming the low temperature and nitrite stress.
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