Related Experiment Video

Updated: Aug 16, 2025

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology
10:25

Screening Traditional Chinese Medicine Compounds for Inhibiting UCHL3 Activity Based on Molecular Docking and Deubiquitinating Enzyme Probe Technology

Published on: November 22, 2024

359

UBE2J1 inhibits colorectal cancer progression by promoting ubiquitination and degradation of RPS3

Tuo Wang1,2,3,4, Chi Jin1,2,3,4, Peng Yang1,2,3,4

  • 1Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.

Oncogene
|December 25, 2022
PubMed

Insights

Ubiquitin-conjugating enzyme E2 J1 (UBE2J1) acts as a tumor suppressor in colorectal cancer (CRC). Downregulation of UBE2J1 promotes CRC progression by stabilizing RPS3 and activating the NF-κB pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ubiquitin-conjugating enzyme E2 J1 (UBE2J1) is involved in protein ubiquitination, but its role in cancer remains unclear.
  • Understanding UBE2J1's function in colorectal cancer (CRC) is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the role of UBE2J1 in colorectal cancer development and progression.
  • To elucidate the molecular mechanisms by which UBE2J1 affects CRC cell behavior.

Main Methods:

  • Analysis of UBE2J1 expression in CRC tissues and cell lines.
  • Investigation of UBE2J1's effect on CRC cell proliferation and metastasis.
  • Identification of UBE2J1's interaction partners and substrates using biochemical assays.
  • Examination of the impact of UBE2J1 on the NF-κB signaling pathway.

Main Results:

  • UBE2J1 is downregulated in CRC tissues and cell lines due to promoter hypermethylation, correlating with poor prognosis.
  • UBE2J1 suppresses CRC cell proliferation and metastasis.
  • UBE2J1, via an E2-E3 complex with TRIM25, targets RPS3 for ubiquitination and degradation at K214.
  • Downregulation of RPS3 by UBE2J1 inhibits NF-κB nuclear translocation and inactivates the NF-κB pathway.

Conclusions:

  • UBE2J1 functions as a tumor suppressor in CRC by promoting RPS3 degradation and inhibiting the NF-κB signaling pathway.
  • UBE2J1-mediated regulation of RPS3 and NF-κB offers a potential therapeutic target and biomarker for CRC.

More Related Videos

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples09:45

Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Published on: May 10, 2015

9.7K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.3K

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
973
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K