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In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Characterization of Blood Outgrowth Endothelial Cells BOEC from Porcine Peripheral Blood
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Blastocyst complementation and interspecies chimeras in gene edited pigs.

Yong-Ho Choe1,2, Jacob Sorensen1,2, Daniel J Garry1,2,3,4

  • 1Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, United States.

Frontiers in Cell and Developmental Biology
|December 26, 2022
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Summary

Creating interspecies chimeric organs using advanced gene editing and stem cell technologies offers a promising solution to the critical shortage of donor organs for transplantation in end-stage diseases.

Keywords:
ETV2MYF6Myf5MyoDblastocyst complementationpigssomatic cell nuclear transfer

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Area of Science:

  • Regenerative Medicine
  • Transplantation Biology
  • Bioengineering

Background:

  • Allograft organ transplantation is the only curative option for end-stage diseases.
  • A significant shortage of donor organs limits transplantation accessibility.
  • Novel strategies are essential to overcome donor organ scarcity.

Purpose of the Study:

  • To review the technical strategies for creating interspecies chimeric organs.
  • To highlight early successful results in chimeric organ development.
  • To identify challenges hindering clinical application of chimeric organs.

Main Methods:

  • Gene editing technologies for precise genetic modification.
  • Somatic cell nuclear transfer for creating genetically modified cells.
  • Human induced pluripotent stem cell (hiPSC) technologies for generating organ precursors.
  • Interspecies chimera generation using these advanced techniques.

Main Results:

  • Demonstration of early promising results in developing chimeric organs.
  • Successful integration of human cells within interspecies environments.
  • Proof-of-concept for generating organoids or organ structures.

Conclusions:

  • Interspecies chimeric organ development shows potential to address donor organ shortage.
  • Further research is needed to overcome technical and biological hurdles.
  • Clinical translation requires rigorous validation and ethical considerations.