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The Origin-Adjusted Approach for Reliable Quantification of Endogenous Analytes in Mass Spectrometric Bioanalysis.

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A novel origin-adjusted (OA) method simplifies biomarker quantification in drug development. This technique, based on standard addition, offers accurate and precise results comparable to existing methods but with reduced complexity and cost.

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Area of Science:

  • Biomarker quantification
  • Bioanalytical chemistry
  • Analytical method development

Background:

  • Accurate biomarker quantification is crucial for drug development and diagnostics.
  • Existing methods like surrogate matrix (SUR-M) and surrogate analyte (SUR-A) have limitations.
  • A need exists for simpler, faster, and more cost-effective quantification techniques.

Purpose of the Study:

  • To introduce a novel origin-adjusted (OA) approach for biomarker quantification.
  • To demonstrate the OA method's advantages over existing techniques.
  • To compare the OA method with the surrogate matrix (SUR-M) approach.

Main Methods:

  • The study adapted the standard addition (SA) method.
  • A key modification involved adjusting the calibration curve's intercept to zero.
  • The novel origin-adjusted (OA) method was applied to kynurenine and tryptophan quantification in human plasma using LC-MS.

Main Results:

  • The origin-adjusted (OA) method effectively removes the influence of endogenous analyte levels.
  • Performance analysis demonstrated equivalence between the OA and SUR-M approaches.
  • The OA method provides a simpler and potentially faster alternative for biomarker quantification.

Conclusions:

  • The origin-adjusted (OA) method is a viable and advantageous technique for biomarker quantification.
  • This novel approach simplifies bioanalytical workflows, particularly for LC-MS applications.
  • The OA method holds significant potential for improving efficiency in drug development and diagnostics.