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Updated: Aug 16, 2025

$Synchrotron X-ray Microdiffraction and Fluorescence Imaging of Mineral and Rock Samples$

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Published on: June 19, 2018

Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections.

Prakash Nepal¹, Abdullah Al Bashit², Lin Yang³

¹Department of Bioengineering, Northeastern University, Boston, MA, USA.

Journal of Applied Crystallography

|December 26, 2022

Summary

This summary is machine-generated.

Small-angle X-ray scattering (SAXS) can now reveal fibril structure and tissue organization. New methods analyze scattering from small tissue volumes, overcoming complex sample limitations.

Keywords:

Alzheimer’s disease SAXS amyloids scanning microdiffraction small-angle X-ray scattering

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$X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease$

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

Published on: July 18, 2019

Area of Science:

Biophysics
Materials Science
Biomolecular Imaging

Background:

Small-angle X-ray scattering (SAXS) from tissue fibrils is complex due to multiple components.
Extracting specific fibril information is challenging due to surrounding matrix and cross-terms.
Micro- and nano-beams enable scattering measurements from smaller, potentially single-component volumes.

Purpose of the Study:

To develop strategies for extracting fibril structure and tissue organization information from SAXS data.
To demonstrate the utility of spatial correlation functions in analyzing complex biological samples.
To overcome limitations of traditional X-ray solution scattering techniques for tissue analysis.

Main Methods:

Utilizing micro- and nano-beams for small-volume SAXS measurements.
Developing methods to compute spatial correlation functions from SAXS data.
Analyzing scattering data to isolate fibril-specific information and tissue organization.

Main Results:

Proposed strategies successfully extract partial information on fibril structure and tissue organization.
The spatial correlation function perpendicular to the fibril axis provides insights into fibril structure and matrix organization.
Demonstrated information retrieval from various sample types using correlation calculations.

Conclusions:

SAXS, with advanced analysis, can provide detailed insights into fibril structure within complex tissue environments.
Spatial correlation functions are a powerful tool for understanding fibril-matrix interactions and tissue architecture.
The proposed methods offer significant advantages over existing X-ray scattering techniques for biological tissues.