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Oestrogen synthesis by the foetal rat testis in organ culture
1Laboratoire de Zoologie et d'Embryologie Expérimentale, Université Louis-Pasteur, Strasbourg, France.
Journal of Steroid Biochemistry
|September 1, 1987
Abstract:
Testes from 19- to 21-day old rat foetuses were bisected and cultured in the presence of tritiated testosterone, androstenedione or dehydroepiandrosterone as precursors for oestrogen biosynthesis. Oestrone and oestradiol formed were identified by recrystallization to constant specific activity, and their conversion rate was determined after isotopic dilution. Both oestrogens formed from either precursor, their conversion rate being about 0.05% for oestradiol and 0.015% for oestrone.