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Updated: May 24, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

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Highly efficient polyhydroxyalkanoate production from lignin using genetically engineered Halomonas sp. Y3.

Yuan-Qiu Li¹, Ming-Jun Wang², Chao-Bing Luo²

¹College of Life Science, Leshan Normal University, Leshan 614000, China; College of Life Sciences, Capital Normal University, Beijing 100064, China.

Bioresource Technology

|December 26, 2022

Summary

This study engineered microbes for efficient lignin conversion into polyhydroxyalkanoates (PHA). It achieved record PHA titers, offering a greener route for valuable chemical production from lignin waste.

Keywords:

Engineering Halomonas Laccase Lignin Polyhydroxyalkanoate

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Area of Science:

Biotechnology
Microbial Engineering
Biorefining

Background:

Lignin degradation is a major hurdle in biological valorization, limiting efficient conversion.
Current methods face biochemical limitations in depolymerizing lignin effectively.

Purpose of the Study:

To develop an efficient laccase secretion system for enhanced lignin depolymerization.
To engineer polyhydroxyalkanoate (PHA) biosynthesis for high-yield production from lignin derivatives.
To establish a microbial platform for sustainable PHA production from lignin.

Main Methods:

Engineered a highly efficient laccase secretion apparatus in Halomonas sp. Y3.
Optimized PHA biosynthesis pathways for increased product yield.
Integrated laccase secretion and PHA production modules.
Developed a non-sterilized fermentation process.

Main Results:

Achieved a high laccase enzyme activity of 184 U/mL.
Produced PHA titers of 286, 742, and 868 mg/L from alkaline lignin, catechol, and protocatechuate.
Reached record PHA titers of 693 and 1209 mg/L from lignin and lignin-containing streams.
Further improved PHA titers to 740 and 1314 mg/L using non-sterilized fermentation.

Conclusions:

The developed microbial platform enables cost-effective and environmentally friendly PHA production from lignin.
This work provides novel insights into lignin conversion by extremophilic microbes.
The engineered system overcomes biochemical limitations in lignin valorization.