Related Experiment Video
Updated: Aug 16, 2025

Evaluation of Photosynthetic Efficiency in Photorespiratory Mutants by Chlorophyll Fluorescence Analysis
Published on: December 9, 2022
Chlorophyll fluorometry in evaluating photosynthetic performance: key limitations, possibilities, perspectives and
Vladimir Lysenko1, Vishnu D Rajput1, Rupesh Kumar Singh2
1Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.
Abstract:
Non-destructive methods for the assessment of photosynthetic parameters of plants are widely applied to evaluate rapidly the photosynthetic performance, plant health, and shifts in plant productivity induced by environmental and cultivation conditions. Most of these methods are based on measurements of chlorophyll fluorescence kinetics, particularly on pulse modulation (PAM) fluorometry. In this paper, fluorescence methods are critically discussed in regard to some their possibilities and limitations inherent to vascular plants and microalgae. Attention is paid to the potential errors related to the underestimation of thylakoidal cyclic electron transport and anoxygenic photosynthesis. PAM-methods are also observed considering the color-addressed measurements. Photoacoustic methods are discussed as an alternative and supplement to fluorometry. Novel Fourier modifications of PAM-fluorometry and photoacoustics are noted as tools allowing simultaneous application of a dual or multi frequency measuring light for one sample.
More Related Videos
10:20Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
07:03Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
Published on: November 12, 2021
Related Concept Videos
Photoluminescence: Applications
Fluorescence and Phosphorescence: Instrumentation
Flame Photometry: Overview
The Calvin Benson Cycle
Variables Affecting Phosphorescence and Fluorescence
Photoreceptors and Plant Responses to Light