A protocol to detect human CD4⁺ T cell extracellular traps using scanning electron microscopy

We present a protocol to detect extracellular traps (ETs) induced by Cutibacterium acnes in cultured T_H17 clones. We first describe the isolation of C. acnes-specific T_H17 clones by sterile cell sorting. We then detail the in vitro induction of ETs in T_H17 clones stimulated by C. acnes and the imaging of released ETs using scanning electron microscopy. This protocol can be applied to the study of other ETs released by other T cell subsets. For complete details on the use and execution of this protocol, please refer to Agak et al. (2021).¹.