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A protocol to detect human CD4+ T cell extracellular traps using scanning electron microscopy.

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Researchers developed a new protocol to detect extracellular traps (ETs) released by TH17 cells in response to Cutibacterium acnes. This method aids in studying T cell-mediated immune responses.

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Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • Cutibacterium acnes (C. acnes) is a bacterium implicated in various inflammatory conditions.
  • TH17 cells play a crucial role in adaptive immunity and host defense.
  • Extracellular traps (ETs) are increasingly recognized as a component of the immune response.

Purpose of the Study:

  • To establish a protocol for detecting extracellular traps (ETs) induced by C. acnes in TH17 clones.
  • To provide a method for studying the interaction between C. acnes and TH17 cells.

Main Methods:

  • Isolation of C. acnes-specific TH17 clones using sterile cell sorting.
  • In vitro induction of ETs in TH17 clones stimulated with C. acnes.
  • Imaging of released ETs via scanning electron microscopy.

Main Results:

  • Successful isolation of specific TH17 clones.
  • Demonstration of ET formation by TH17 cells upon stimulation with C. acnes.
  • Visualization of ETs using scanning electron microscopy.

Conclusions:

  • The presented protocol enables the detection and study of C. acnes-induced ETs in TH17 cells.
  • This methodology can be extended to investigate ETs from other T cell subsets.
  • The findings contribute to understanding T cell-mediated immune responses to bacterial stimuli.