Related Experiment Video
Updated: Aug 16, 2025
09:40
Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
2.8K
Circ_0006220 Contributes to NSCLC Progression through miR-342-3p/GOT2 Axis
Jichun Tang1, Xuan Li1, Lili Zhao1
1Department of Medical of Oncology, Wuxi Huishan District People's Hospital, Wuxi, Jiangsu, China.
Summary
Circular RNA circ_0006220 promotes non-small cell lung cancer (NSCLC) progression by regulating the microRNA-342-3p/GOT2 pathway. Silencing circ_0006220 inhibits tumor cell proliferation, migration, and angiogenesis while promoting apoptosis in NSCLC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) play a role in cancer development.
- Dysregulated circRNAs are implicated in non-small cell lung cancer (NSCLC) tumorigenesis.
Purpose of the Study:
- To investigate the biological functions of circ_0006220 in NSCLC.
- To elucidate the regulatory mechanisms of circ_0006220 in NSCLC progression.
Main Methods:
- Quantitative PCR and Western blot for expression analysis.
- Cell proliferation, apoptosis, migration, and angiogenesis assays in NSCLC cell lines.
- Dual-luciferase reporter and RNA immunoprecipitation assays to confirm molecular interactions.
Main Results:
- Circ_0006220 is upregulated in NSCLC tissues and cell lines.
- Circ_0006220 knockdown suppresses NSCLC cell proliferation, migration, invasion, and angiogenesis, while inducing apoptosis.
- Circ_0006220 acts as a miR-342-3p sponge, regulating the miR-342-3p/GOT2 axis.
Conclusions:
- Circ_0006220 promotes NSCLC malignancy via the miR-342-3p/GOT2 pathway.
- Circ_0006220 may serve as a potential therapeutic target for NSCLC.
More Related Videos
Related Concept Videos
01:20The Retinoblastoma Gene
4.2K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
03:03mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
02:07Tumor Progression
6.4K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.4K
01:22MicroRNAs
3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:33Cancer-Critical Genes I: Proto-oncogenes
9.1K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.1K