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Related Experiment Video

Updated: Aug 16, 2025

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.5K

High throughput mutagenesis and screening for yeast engineering.

Kendreze Holland1,2, John Blazeck3,4

  • 1Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.

Journal of Biological Engineering
|December 27, 2022
PubMed
Summary
This summary is machine-generated.

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This review covers advancements in yeast engineering, focusing on genome-wide screening platforms and high-throughput methods for improving cellular functions and biomolecules. Future developments promise further progress in yeast high-throughput engineering.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Synthetic Biology

Background:

  • * Saccharomyces cerevisiae is a key model organism for biocatalysis and disease research.
  • * Recent years have seen rapid growth in yeast-based studies due to new genetic tools.

Purpose of the Study:

  • * To review genome-scale screening platforms and genetic mutagenesis tools in yeast.
  • * To highlight advances in biomolecule evolution and high-throughput selection strategies.
  • * To discuss future directions in yeast high-throughput engineering.

Main Methods:

  • * Review of genome-wide screening platforms, including CRISPR/Cas.
  • * Discussion of methods for rapid and continuous biomolecule evolution.
  • * Examination of high-throughput selection and screening strategies.
Keywords:
CRISPR-Cas9Cellular engineeringDirected evolutionGenome-wideHigh throughputIn vivo mutagenesisProtein engineeringS. cerevisiaeSelection schemesYeast-display

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Related Experiment Videos

Last Updated: Aug 16, 2025

High-throughput Yeast Plasmid Overexpression Screen
08:57

High-throughput Yeast Plasmid Overexpression Screen

Published on: July 27, 2011

16.5K
High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

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Mating-based Overexpression Library Screening in Yeast
11:39

Mating-based Overexpression Library Screening in Yeast

Published on: July 6, 2018

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Main Results:

  • * Detailed overview of past and present genome-scale screening platforms.
  • * Highlighted recent advances in evolving biomolecules for enhanced function.
  • * Identified essential high-throughput strategies for assessing cellular and protein improvements.

Conclusions:

  • * Yeast engineering has significantly advanced with new tools and strategies.
  • * High-throughput methods are crucial for isolating improvements in yeast.
  • * Future developments will continue to drive innovation in yeast high-throughput engineering.