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OsPPR11 encoding P-type PPR protein that affects group II intron splicing and chloroplast development
Qiang Zhang1, Changzhao Chen1, Yaliang Wang1
1State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, People's Republic of China.
Plant Cell Reports
|December 28, 2022
Summary
Rice chloroplast development relies on OsPPR11, a P-type pentatricopeptide repeat (PPR) protein. OsPPR11 regulates Group II intron splicing and interacts with OsCAF2, crucial for plant growth and photosynthesis.
Area of Science:
- Plant Biology
- Molecular Genetics
- Chloroplast Biology
Background:
- Pentatricopeptide repeat (PPR) proteins are vital for organelle gene expression in plants.
- Many PPR proteins in rice remain functionally uncharacterized.
- PPR proteins are known to be involved in Group II intron splicing within chloroplasts and mitochondria.
Purpose of the Study:
- To identify and characterize a novel P-type PPR protein, OsPPR11, involved in chloroplast function in rice.
- To investigate the role of OsPPR11 in Group II intron splicing and chloroplast development.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
- Phenotypic analysis of osppr11 mutants.
- Analysis of chloroplast development and photosynthetic gene/protein expression.
- Protein-protein interaction studies (co-immunoprecipitation).
Main Results:
- OsPPR11 is expressed in all rice tissues, with highest levels in leaves.
- osppr11 mutants exhibit yellowing leaves, developmental defects, and reduced photosynthesis.
- OsPPR11 is essential for the splicing of specific Group II introns (ndhA and ycf3-1).
- OsPPR11 interacts with OsCAF2, a protein from the CRM family.
Conclusions:
- OsPPR11 is a critical nuclear-encoded P-type PPR protein essential for rice chloroplast development and function.
- OsPPR11 regulates Group II intron splicing, likely in complex with OsCAF2.
- Defects in OsPPR11 lead to severe disruptions in chloroplast biogenesis and photosynthesis.
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