Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.1K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.1K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

776
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
776
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.1K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.1K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

261
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
261
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

264
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
264
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

1.6K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The dual-faced pyruvate kinase M2 in tumors: From cytoplasmic metabolic gatekeeper to nuclear transcriptional coactivator.

Biochemical pharmacology·2026
Same author

Integrating Binding Thermodynamics and Relaxation for Evaluating Substrate-Dependent SABRE Performance.

Analytical chemistry·2026
Same author

Leveraging the Oryza telomere-to-telomere genome and wild-rice substitution lines for rice-quality improvement.

Current biology : CB·2026
Same author

An in-sensor communication electronic textile for imperceptible and ultrarobust silent speech.

Nature communications·2026
Same author

FlexCENT: A frequency-flexible CEST imaging network combining frequency offset encoding and three-dimensional U-Net.

Magnetic resonance letters·2026
Same author

Ti Substitution-Induced Inter-Site Distance Effect (ISDE) in Hierarchical Porous Cobalt Titanium Oxide Promoting Selective NH<sub>3</sub> Oxidation for Enhanced Energy Release.

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: Aug 15, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.5K

A Pure Shift-Based Nuclear Magnetic Resonance Method for In-Phase Three-Dimensional Diffusion-Ordered Spectroscopy.

Haolin Zhan1,2, Mengyou Hao1, Enping Lin1

  • 1Department of Electronic Science, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, Fujian 361005, China.

Analytical Chemistry
|December 29, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces an in-phase multidimensional DOSY strategy to overcome spectral congestion in complex mixture analysis. The new method enhances resolution, revealing diffusion behaviors and multiplet structures for better component identification.

More Related Videos

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K
Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.0K

Related Experiment Videos

Last Updated: Aug 15, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.5K
High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

5.7K
Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.0K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Physical Chemistry

Background:

  • Diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY) is crucial for analyzing mixtures.
  • Spectral congestion in DOSY limits its application to complex samples due to J-coupling and numerous compounds.

Purpose of the Study:

  • To develop an in-phase multidimensional DOSY strategy for enhanced complex mixture analysis.
  • To simultaneously resolve molecular diffusion and multiplet structures with improved spectral resolution.

Main Methods:

  • Development of pure shift-based three-dimensional (3D) DOSY protocols.
  • Implementation of an in-phase multidimensional DOSY strategy.

Main Results:

  • Achieved high-resolution 3D spectroscopic views for complex mixtures.
  • Successfully separated mixture components and resolved their multiplet coupling structures.
  • Demonstrated utility even under adverse magnetic field conditions.

Conclusions:

  • The proposed strategy offers a promising tool for analyzing complex mixtures.
  • Enables detailed component analysis and multiplet structure studies in practical samples.