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Hsa_circ_0049396 inhibited oral squamous cell carcinoma progression by regulating the miR-663b/ENDOU axis

Ying Lou1, Liuyang Ren1, Lu Wang1

  • 1Department of Stomatology, Henan Provincial People's Hospital, Zhengzhou 450003, Henan, China.

Genomics
|December 29, 2022
PubMed
Abstract

Insights

Circular RNA (circRNA) circ_0049396 inhibits oral squamous cell carcinoma (OSCC) progression by regulating the miR-663b/ENDOU axis. Overexpression of circ_0049396 shows potential as a therapeutic strategy for OSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer progression.
  • Oral squamous cell carcinoma (OSCC) is a significant global health concern.
  • Understanding novel circRNAs like circ_0049396 is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the function of circ_0049396 in oral squamous cell carcinoma (OSCC).
  • To elucidate the underlying molecular mechanism of circ_0049396 in OSCC progression.
  • To evaluate circ_0049396 as a potential therapeutic target for OSCC.

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) for gene expression analysis.
  • Cellular assays (CCK-8, Transwell) for proliferation and migration.
  • Western blotting for apoptosis-related proteins.
  • In vivo xenograft models for tumorigenesis assessment.
  • Molecular interaction studies (dual luciferase, RNA pull-down, RIP assays).

Main Results:

  • circ_0049396 and ENDOU were downregulated, while miR-663b was upregulated in OSCC.
  • Overexpression of circ_0049396 suppressed OSCC cell proliferation, migration, and tumorigenesis, while enhancing apoptosis.
  • The circ_0049396/miR-663b/ENDOU regulatory network was experimentally validated.
  • MiR-663b promoted OSCC cell malignancy, and circ_0049396/ENDOU overexpression partially reversed these effects.

Conclusions:

  • circ_0049396 inhibits OSCC malignant behavior by modulating the miR-663b/ENDOU axis.
  • circ_0049396 demonstrates potential as a therapeutic agent for oral squamous cell carcinoma.
  • Targeting the circ_0049396/miR-663b/ENDOU pathway could offer novel treatment strategies for OSCC.
Keywords:
ENDOUOral squamous cell carcinomaProliferationcirc_0049396miR-663b

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