Related Experiment Video

Updated: Aug 15, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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RNF31 represses cell progression and immune evasion via YAP/PD-L1 suppression in triple negative breast Cancer

Huijie Yang1, Min Xue2, Peng Su3

  • 1Xinxiang Key Laboratory of Tumor Migration and Invasion Precision Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang, 453003, Henan Province, People's Republic of China.

Journal of Experimental & Clinical Cancer Research : CR
|December 29, 2022
PubMed
Abstract

Insights

RING finger protein 31 (RNF31) suppresses tumors in triple-negative breast cancer (TNBC) by inhibiting the Hippo/YAP pathway. Activating RNF31 may offer a new therapeutic strategy for TNBC, potentially improving immunotherapy response.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Signaling Pathways

Background:

  • Hippo signaling pathway abnormalities are common in triple-negative breast cancer (TNBC) and drive cancer progression.
  • RING finger protein 31 (RNF31), an E3 ubiquitin ligase, is known to be elevated in ER-positive breast cancer.
  • Previous research indicated RNF31's role in activating estrogen signaling and suppressing the P53 pathway in ER-positive breast cancer.

Purpose of the Study:

  • To investigate the function of RNF31 in the progression of triple-negative breast cancer (TNBC).
  • To explore the potential of RNF31 as a therapeutic target for TNBC.

Main Methods:

  • Utilized TNBC cell lines and xenograft models for in vitro and in vivo studies.
  • Performed immuno-blots, QPCR, and global genomic expression profiling.
  • Conducted molecular biology assays to examine protein interactions and ubiquitination.

Main Results:

  • RNF31 exhibits a tumor-suppressive function in TNBC, with RNF31 depletion increasing cell proliferation and migration.
  • RNF31 depletion upregulates YAP protein and Hippo signaling target genes, indicating Hippo signaling as a target.
  • RNF31 expression correlates with longer relapse-free survival in TNBC patients and represses PDL1 expression, sensitizing TNBC to immunotherapy via the Hippo/YAP/PDL1 axis.

Conclusions:

  • RNF31 plays a multifaceted role in different breast cancer subtypes.
  • Activation of RNF31 presents a potential therapeutic strategy for TNBC.
  • Targeting the Hippo/YAP/PDL1 axis through RNF31 modulation could enhance TNBC immunotherapy outcomes.
Keywords:
Immune evasionPD-L1RNF31StabilityTNBCYAP

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