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Association of eNOS gene 4a/4b VNTR and T786C polymorphism with Crimean-Congo hemorrhagic fever

Umut Safiye Say Coskun1, Serbulent Yigit2, Zeliha Cansel Ozmen3

  • 1Department of Medical Microbiology, Faculty of Medicine, Tokat Gaziosmanpasa University, Tokat, Turkey.

Nucleosides, Nucleotides & Nucleic Acids
|December 30, 2022
PubMed

Insights

Genetic variations in the endothelial nitric oxide synthase (eNOS) T786C gene may increase susceptibility to Crimean-Congo hemorrhagic fever (CCHF). This study found significant differences in eNOS T786C allele and genotype frequencies between CCHF patients and healthy controls.

Area of Science:

  • Genetics
  • Virology
  • Molecular Biology

Background:

  • Crimean-Congo hemorrhagic fever (CCHF) is a severe viral illness with significant mortality.
  • Endothelial nitric oxide synthase (eNOS) gene polymorphisms are implicated in various viral diseases and hemorrhagic conditions.
  • Understanding genetic factors influencing CCHF susceptibility is crucial for disease management.

Purpose of the Study:

  • To investigate the association between eNOS gene 4a/4b and T786C polymorphisms and CCHF.
  • To determine if specific eNOS genotypes or alleles are risk factors for developing CCHF.

Main Methods:

  • Genotyping of eNOS 4a/4b and T786C polymorphisms using polymerase chain reaction and restriction fragment length polymorphism.
  • Analysis of CCHF virus RNA and DNA isolation from patient samples.
  • Statistical comparison of genotype and allele frequencies between 54 CCHF patients and 60 control subjects.

Main Results:

  • No statistically significant difference was observed in the frequencies of eNOS 4a/4b genotypes between CCHF patients and controls.
  • A statistically significant difference (p < 0.05) was found in the allele and genotype frequencies of the eNOS T786C variant between CCHF patients and the control group.
  • The T786C polymorphism showed distinct distribution patterns in CCHF patients compared to controls.

Conclusions:

  • The eNOS T786C gene variant may act as a genetic determinant for CCHF susceptibility.
  • This is the first study to report a potential link between eNOS T786C polymorphisms and CCHF.
  • Further research is warranted to elucidate the precise role of eNOS in CCHF pathogenesis.

Keywords:
Crimean Congo Hemorrhagic FevereNOS gene 4a4beNOS gene T786Cgene polymorphisms

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