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LQB-118 Suppresses Migration and Invasion of Prostate Cancer Cells by Modulating the Akt/GSK3β Pathway and MMP-9/Reck

Thiago Martino1, Graziele Freitas DE Bem2, Shirley Vania Moura Santos1

  • 1LIA-BPPN, Department of Biochemistry, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil.

Anticancer Research
|December 30, 2022
PubMed
Abstract

Insights

LQB-118, a novel compound, inhibits prostate cancer (PCa) cell migration and invasion. It targets the Akt/GSK3 signaling pathway, offering potential for treating metastatic PCa.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Prostate cancer (PCa) is a prevalent malignancy in men.
  • LQB-118, a pterocarpanquinone, exhibits antitumor properties against PCa cells.
  • Previous studies showed LQB-118 inhibits proliferation; its effect on migration was unknown.

Purpose of the Study:

  • To evaluate the effects of LQB-118 on prostate cancer cell migration and invasion.
  • To elucidate the underlying mechanisms of LQB-118's action on PCa metastasis.

Main Methods:

  • PC3 cells were treated with LQB-118 or Paclitaxel.
  • Cell migration and invasion assays (wound healing, Boyden chamber, Matrigel) were performed.
  • Mechanisms were assessed via flow cytometry, Western blot, and qPCR for protein expression, phosphorylation, and mRNA levels.

Main Results:

  • LQB-118 significantly impaired PCa cell migration and invasion.
  • It down-regulated Akt and GSK3β phosphorylation via a FAK-independent pathway.
  • LQB-118 modulated MMP-9 and Reck mRNA levels, and affected integrin αvβIII expression without increasing cell adhesion.

Conclusions:

  • LQB-118 negatively regulates the Akt/GSK3 signaling pathway in prostate cancer cells.
  • It modulates PCa cell proliferation, death, and metastasis.
  • LQB-118 demonstrates pleiotropic activities, supporting its potential use in treating metastatic PCa.
Keywords:
AktMMP-9Pterocarpanquinoneprostate cancer

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