Cu²⁺-mediated turn-on fluorescence biosensor based on DNA-templated silver nanoclusters for label-free and sensitive...

A Cu²⁺-mediated turn-on fluorescence biosensor based on the DNA-templated green-emitting silver nanoclusters (DNA@g-AgNCs) was developed for label-free and sensitive detection of adenosine 5'-triphosphate (ATP). Cu²⁺ was able to quench the bright green fluorescence of DNA@g-AgNCs because of the coordination and photoinduced electron transfer between DNA@g-AgNCs and Cu²⁺. Therefore, a unique and effective fluorescence biosensor can be constructed with the formation of DNA@g-AgNCs/Cu²⁺/ATP ternary-competition system. With the introduction of ATP, the DNA@g-AgNCs/Cu²⁺ fluorescence sensing system will be disrupted and the fluorescence of DNA@g-AgNCs was recovered due to higher affinity of ATP towards Cu²⁺. On the basis of this feature, the DNA@g-AgNCs/Cu²⁺ fluorescence sensing system demonstrated quantitative determination of ATP in the range 0.05 - 3 μM and a detection limit of 16 nM. Moreover, the fluorescence sensing system was successfully applied to the quantitative determination of ATP in human urine and serum samples with recoveries ranging from 98.6 to 106.5%, showing great promise to provide  a label-free, cost-efficient, and rapid platform for ATP-related clinical disease diagnosis.