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Updated: Jul 27, 2026

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
Published on: March 26, 2019
The equivalent background of bleaching
1Department of Zoology, University of Cambridge, UK.
Abstract:
Stiles and Crawford proposed that a retinal region bleached by preexposure to intense light behaves as if it were illuminated by some steady veiling or background luminance. We test this notion by comparing the afterimage of a bleaching light with a steady (and retinally stabilized) light of adjustable intensity, in the manner of Barlow and Sparrock. With their matching procedure, and also with a new procedure, we find as they did that during the rod phase of recovery the afterimage does look like a stabilized field of an intensity which, presented as a background, brings visual sensitivity to the same level. It is as if the two conditions produce equal signals at some stage of the visual pathway. Liked Barlow and Sparrock we observe a rod-cone break in the afterimage matches. However, we argue that the appearance of the rod-cone break presents a paradox and we show a way to resolve it.
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