Dual-key-and-lock AIE probe for thiosulfate and Ag⁺ detection in mitochondria

Ag⁺ ion detection has attracted much attention due to its important role in chemical and biological processes, as well as its potential threat to the environment and human health. Herein, we firstly constructed a dual-key-and-lock sensing strategy for Ag⁺ detection based on three-component co-assembly. An aggregation-induced emission luminogen (AIEgen), namely triphenylamine-thiophene-pyridinium (abbreviated to TPA-T-Py), showed unique co-assembly capability with Ag⁺ and S₂O₃^2- in PBS buffer (pH 7.4, 0.01 M). Cell imaging further proved that mitochondria can be lit up by TPA-T-Py under the dual key stimulation, which was successfully used for Ag⁺ and S₂O₃^2- detection in vitro. In brief, we provide a promising strategy for the construction of dual-lock imaging agents with organelle-targeting ability.