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The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions.

Fabienne Mauxion1, Jérôme Basquin2, Sevim Ozgur2

  • 1Institut de Génétique et de Biologie Moléculaire et cellulaire (IGBMC), Centre National de Recherche scientifique (CNRS) UMR 7104 - Institut National de santé et de Recherche Médicale (Inserm) U964 - Université de Strasbourg, 1 rue Laurent Fries, Illkirch, France.

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|December 31, 2022
PubMed
Summary
This summary is machine-generated.

The CCR4-NOT complex

Keywords:
AlphaFoldCCR4-NOT complexCP: Molecular biologyGGNBP2X-ray crystallographycryo-EMdeadenylationmRNA decayprotein-protein interactions

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The CCR4-NOT complex is a crucial cytoplasmic factor regulating mRNA turnover and decay.
  • Its modular structure allows for versatile functions in gene expression control.
  • The N-terminal module's architecture and function remained largely uncharacterized.

Purpose of the Study:

  • To elucidate the high-resolution structure of the human N-terminal CCR4-NOT module (CNOT1, CNOT10, CNOT11).
  • To identify and characterize novel interacting partners of this module.
  • To understand the role of the N-terminal module as a protein-protein interaction platform.

Main Methods:

  • Employed various structural approaches to determine high-resolution data.
  • Utilized biochemical analyses to confirm protein interactions.
  • Focused on the structural elucidation of the CNOT1-CNOT10-CNOT11 complex.

Main Results:

  • Revealed the architecture of the N-terminal module, showing CNOT1 sandwiching CNOT10 and CNOT11.
  • Identified GGNBP2 as a conserved interacting partner of the CNOT11 'antenna' domain.
  • Demonstrated the N-terminal module's function as a conserved protein-protein interaction hub.

Conclusions:

  • The N-terminal CNOT1-CNOT10-CNOT11 module serves as a key platform for protein interactions.
  • The CNOT11 antenna domain mediates interactions with proteins like GGNBP2.
  • This structural insight advances understanding of CCR4-NOT complex regulation and function.