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Updated: Aug 15, 2025

Investigating Single Molecule Adhesion by Atomic Force Spectroscopy
Published on: February 27, 2015
Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single-Molecule Atomic Force
Andrew Vae Priest1, Sanjeevi Sivasankar2
1Department of Biomedical Engineering, University of California, Davis, CA, USA.
Abstract:
Cell adhesion proteins play essential roles in the formation, regeneration, and maintenance of tissue. However, the molecular mechanisms by which cells regulate the conformation and binding properties of adhesion proteins are poorly understood. These biophysical properties can be resolved, with single-molecule resolution, using atomic force microscopy (AFM). Here, we outline how AFM force measurements can be used to study the conformation, cytoskeletal linkage, binding strength, and force-dependent bond lifetimes of adhesion proteins in live cells.
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