Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Mitochondrial fission links ECM mechanotransduction to metabolic redox homeostasis and metastatic chemotherapy resistance.

Nature cell biology·2026
Same author

Tau20-RETROmapping System Identifies Driver-Like Activation Patterns in Real-Time During Persistent Atrial Fibrillation.

Circulation. Arrhythmia and electrophysiology·2026
Same author

Delayed Sequence Intubation and Its Role in Prehospital Emergency Medicine: A Scoping Review.

Air medical journal·2026
Same author

The 2025 AUA Guideline Update on Recurrent Urinary Tract Infections: Important New Recommendations Reflecting Progress in an Often-Ignored Disease Space.

The Journal of urology·2026
Same author

Dynamin 2 Regulates Mitochondrial Mitotic Fission in Pulmonary Hypertension.

Circulation research·2026
Same author

CSF1R Inhibition with Chemotherapy Relieves Systemic Immune Suppression in Patients with Metastatic Triple-Negative Breast Cancer and Boosts anti-PD-1 Efficacy in Transgenic Mammary Tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

Related Experiment Video

Updated: Aug 15, 2025

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
08:25

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

262

Imaging Mass Cytometry in Immuno-Oncology.

Tiziana Cotechini1, Oliver Jones2, Charles Colin Thomas Hindmarch3

  • 1Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. t.cotechini@queensu.ca.

Methods in Molecular Biology (Clifton, N.J.)
|December 31, 2022
PubMed
Summary

Imaging mass cytometry (IMC) allows simultaneous detection of up to 50 markers in fixed tissues. This multiplex imaging technique provides spatially resolved insights into the tumor-immune microenvironment (TiME) for research.

Keywords:
Hyperion™Imaging mass cytometryImmunologyIn situMultiplex immunohistochemistryPathologySpatial profilingTumor microenvironment

More Related Videos

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.2K
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.0K

Related Experiment Videos

Last Updated: Aug 15, 2025

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma
08:25

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

262
Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

17.2K
Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment
06:05

Author Spotlight: Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

Published on: June 2, 2023

8.0K

Area of Science:

  • Biomedical research
  • Immunology
  • Oncology

Background:

  • Accurate profiling of the tumor-immune microenvironment (TiME) is crucial for understanding cancer biology and developing effective therapies.
  • Simultaneous detection of multiple proteins within the TiME is essential for comprehensive analysis.

Purpose of the Study:

  • To provide an overview of the imaging mass cytometry (IMC) workflow for in situ profiling of the TiME.
  • To highlight the potential of IMC in pre-clinical and clinical research.

Main Methods:

  • Overview of the IMC workflow, including sample fixation, multiplex panel design, tissue staining, and data analysis.
  • Utilizing the Hyperion™ imaging system for multiplex imaging.

Main Results:

  • IMC enables detection of up to 50 markers at subcellular resolution on fixed tissues.
  • Provides spatially resolved information critical for understanding the TiME.

Conclusions:

  • IMC is a powerful multiplex imaging modality for detailed analysis of the TiME.
  • The presented workflow facilitates the application of IMC in both pre-clinical and clinical settings.