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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Published on: September 4, 2019

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A CRISPR Platform for Targeted In Vivo Screens

Vincent Maranda1, Yue Zhang1, Frederick S Vizeacoumar2

  • 1Division of Oncology, College of Medicine, University of Saskatchewan, Saskatoon, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|December 31, 2022
PubMed

Insights

This chapter outlines how to perform in vivo CRISPR knockout screens in cancer models. These genetic screens help identify therapeutic targets and understand cancer biology, immunity, and treatment responses.

Area of Science:

  • Genomics
  • Cancer Biology
  • Immunology

Background:

  • Large-scale genetic screens are crucial for identifying cancer therapeutic targets.
  • CRISPR technology has significantly advanced loss-of-function genetic screens.
  • In vivo screens offer unique insights into the tumor microenvironment and cancer immunity.

Purpose of the Study:

  • To present a procedural framework for conducting in vivo CRISPR knockout screens in cancer models.
  • To detail associated notes for optimizing these screens.
  • To highlight the application of these screens in studying cancer biology, anti-tumor immunity, and treatment response prediction.

Main Methods:

  • In vivo CRISPR knockout screens in established cancer models.
  • Detailed procedural framework and practical notes.
  • Focus on genetic interaction identification and functional genomics.

Main Results:

  • Provides a comprehensive guide for researchers.
  • Enables the study of complex biological systems in vivo.
  • Facilitates the discovery of novel cancer-specific genetic interactions.

Conclusions:

  • In vivo CRISPR screens are powerful tools for cancer research.
  • This framework supports the investigation of cancer biology, immunity, and treatment responses.
  • Optimized protocols are essential for successful in vivo genetic screening.
Keywords:
CRISPR-Cas9 screenIn vivo screenPooled screenSynthetic lethality

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