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Updated: Aug 15, 2025

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Enhanced ultrasonic spray ionization for direct mass spectrometry analysis of aqueous solution and complex samples
Debo Wu1, Ziyang Tang1, Lulu Dong2
1Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, East China University of Technology, Guanglan Avenue 418, 330013, Nanchang, PR China.
Abstract:
An efficient and superior soft ionization approach for direct mass spectrometry analysis of a variety of samples such as aqueous solution, raw biological sample and proteins, was developed based on commercially available piezoelectric atomizers. A single conical orifice (5 μm in diameter) was created on the atomizer, which resulted in generation of uniform fine droplets and long-duration of MS signal. The two electrodes of piezoelectric atomizer were connected to the two sides of ceramic ring which was insulated from the metallic substrate. The unique design allowed an additional high voltage input towards the spray reagents, which facilitated direct analysis of more complex samples without sample pre-treatment, such as biological samples (tomato tissue). The ionization was driven by an extremely low electrical power (3.5 V rechargeable battery) yet providing an efficient and superior soft ionization. The method displayed a better thermal and pH stability than nano electrospray ionization (nanoESI) and electrospray ionization (ESI) on direct analysis of Vitamin B and protein aqueous solutions. Quantitative analysis of Vitamin B and Rhodamine B aqueous solutions was also investigated, showing a good linearity (R2 > 0.99). In addition, our results suggested that compared with ESI and nanoESI, the method not only could be used for direct analysis of intact protein, but also provide more information concerning the association between intact protein and the subunits.
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